Comparison of conventional coproscopical methods and commercial coproantigen ELISA kits for the detection of Giardia and Cryptosporidium infections in dogs and cats

Berl Munch Tierarztl Wochenschr. 2004 Sep-Oct;117(9-10):410-3.

Abstract

Faecal samples of 270 dogs and 100 cats from 3 animal shelters in Germany were comparatively examined using conventional coproscopical methods and commercial coproantigen ELISA kits for the detection of Giardia and Cryptosporidium infections. Giardia cysts were found in 9.5% and 0% of the faecal samples in dogs and cats, respectively, examined once using the ZnCl2-NaCl flotation. However, the Giardia coproantigen ELISA (ProSpecT Giardia Microplate Assay) was positive in 29.5% and 22.4% of the samples from dogs and cats, respectively. Direct faecal smears stained with carbol fuchsin showed Cryptosporidium oocysts in one dog (0.4%) and one cat (1%). In contrast, the Cryptosporidium coproantigen ELISA (ProSpecT Cryptosporidium Microplate Assay) reacted positively in 23% of the samples from dogs and 30% of the samples from cats. Both coproantigen ELISAs were more often positive in coproscopically Giardia-negative canine faecal samples that contained Isospora burrowsi/ohioensis oocysts than in faecal samples without any parasite stage. Possible reasons for these observations are discussed.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antigens, Protozoan / analysis
  • Cat Diseases / diagnosis*
  • Cat Diseases / parasitology
  • Cats
  • Cryptosporidiosis / diagnosis
  • Cryptosporidiosis / veterinary*
  • Cryptosporidium / immunology
  • Cryptosporidium / isolation & purification
  • Dog Diseases / diagnosis*
  • Dog Diseases / parasitology
  • Dogs
  • Enzyme-Linked Immunosorbent Assay / methods
  • Enzyme-Linked Immunosorbent Assay / veterinary*
  • Feces / parasitology
  • Giardia / immunology
  • Giardia / isolation & purification
  • Giardiasis / diagnosis
  • Giardiasis / veterinary*
  • Reagent Kits, Diagnostic / veterinary
  • Sensitivity and Specificity

Substances

  • Antigens, Protozoan
  • Reagent Kits, Diagnostic