Optimal conditions for the retrieval of CD4 and CD8 antigens in formalin-fixed, paraffin-embedded tissues

Seok H Kim; Myeong C Kook; Hyung G Song

doi:10.1023/b:hijo.0000039856.43632.66

Optimal conditions for the retrieval of CD4 and CD8 antigens in formalin-fixed, paraffin-embedded tissues

J Mol Histol. 2004 May;35(4):403-8. doi: 10.1023/b:hijo.0000039856.43632.66.

Authors

Seok H Kim¹, Myeong C Kook, Hyung G Song

Affiliation

¹ Department of Pathology, Chungbuk National University College of Medicine, 62 Kaesin-dong, Cheongju, Chungbuk, South Korea.

PMID: 15503814
DOI: 10.1023/b:hijo.0000039856.43632.66

Abstract

The effects of buffer, NaCl, EDTA, and urea on the retrieval of CD4 and CD8 antigens in formaldehyde-fixed, paraffin-embedded tissues with a microwave pressure-cooker were evaluated. The optimal retrieval conditions were found to be borate buffer at pH 8 containing 1 mM NaCl and 1 mM EDTA. Urea was found to be less effective.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CD4 Antigens / chemistry*
CD8 Antigens / chemistry*
Epitopes
Formaldehyde
Humans
Immunohistochemistry / methods*
Lymph Nodes / chemistry*
Paraffin Embedding
Staining and Labeling / methods*
Tissue Fixation

Substances

CD4 Antigens
CD8 Antigens
Epitopes
Formaldehyde