A novel K+ channel with unique localizations in mammalian brain: molecular cloning and characterization

Neuron. 1992 Mar;8(3):473-81. doi: 10.1016/0896-6273(92)90275-i.


Using a cDNA library prepared from circumvallate papillae of rat tongue, we have identified, cloned, and sequenced a novel K+ channel, designated cdrk. The cdrk channel appears to be a member of the Shab subfamily, most closely resembling drk1. Electrophysiologic analysis of expressed cdrk channels reveals delayed rectifier properties similar to those of drk1 channels. Localizations of cdrk mRNA in rat brain and peripheral tissues, assessed by in situ hybridization and Northern blot analysis, differ from any other reported K+ channels. In the brain cdrk mRNA is most concentrated in granule cells of the olfactory bulb and cerebellum. In peripheral tissues, mRNAs for cdrk and drk1 are reciprocally localized, indicating that the K+ channel properties contributed by mammalian Shab homologs may be important in a variety of excitable tissues.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Brain / metabolism*
  • Cloning, Molecular
  • DNA / genetics
  • Gene Expression
  • Genes
  • Ion Channel Gating
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Oligodeoxyribonucleotides / chemistry
  • Polymerase Chain Reaction
  • Potassium Channels / genetics*
  • Potassium Channels / physiology
  • RNA, Messenger / genetics
  • Rats
  • Shab Potassium Channels
  • Tissue Distribution


  • Kcnb2 protein, rat
  • Oligodeoxyribonucleotides
  • Potassium Channels
  • RNA, Messenger
  • Shab Potassium Channels
  • DNA

Associated data

  • GENBANK/M77482