MHC class II-related genes expression in porcine-serum-induced rat hepatic fibrosis

Exp Mol Pathol. 2004 Dec;77(3):214-21. doi: 10.1016/j.yexmp.2004.08.001.


Genes, especially MHC class II-related genes, expression was examined in porcine-serum (PS)-induced hepatic fibrosis model. Brown-Norway (BN) and Wistar rats were injected intraperitoneally with 0.5 ml sterile porcine serum twice a week for 1, 2, 3, 4, and 8 weeks. Histopathologically, inflammation started at 2 weeks in BN and at 4 weeks in Wistar rats, and hepatic fibrosis developed at 4 weeks in BN rats and at 8 weeks in Wistar rats. Microarray analysis done at 2 and 4 weeks revealed that the expression of MHC class II-related genes and acute phase inflammation-related genes significantly increased at 2 weeks in BN and at 4 weeks in Wistar rats. On the other hand, the expression of some transcription-related genes was down-regulated in both strains. In BN rats, the results of semiquantitative RT-PCR analysis done on four MHC class II-related genes mRNAs corresponded well with those of microarray analysis. MHC class II is considered to be involved in the initiation of PS-induced hepatic fibrosis in rats.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Disease Models, Animal
  • Gene Expression*
  • Genes, MHC Class II*
  • Liver / pathology
  • Liver Cirrhosis, Experimental / genetics*
  • Liver Cirrhosis, Experimental / immunology
  • Liver Cirrhosis, Experimental / pathology
  • Male
  • Oligonucleotide Array Sequence Analysis
  • Organ Size
  • Rats
  • Rats, Inbred BN
  • Rats, Wistar
  • Serum / immunology
  • Species Specificity
  • Swine* / blood
  • Swine* / immunology