Isolation of anti-ulcerogenic sesquiterpene lactones from Centaurea solstitialis L. ssp. solstitialis through bioassay-guided fractionation procedures in rats

J Ethnopharmacol. 2004 Dec;95(2-3):213-9. doi: 10.1016/j.jep.2004.07.021.

Abstract

The fresh spiny flowers of Centaurea solstitialis ssp. solstitialis (CSS) are used for the treatment of peptic ulcers in Turkey. Ethanol (80%) extract of CSS exhibited significant anti-ulcerogenic effect on the ethanol-induced ulcerogenesis model in rats. The ethanol extract was further fractionated by successive solvent extractions with n-hexane, chloroform, ethyl acetate and n-butanol. All fractions showed significant anti-ulcerogenic activity, however, the effect of the chloroform fraction was found to be more prominent with 99.5% ulcer inhibition. Bioassay-guided fractionation yielded sesquiterpene lactones as the active components. The main components responsible for the activity of the chloroform fraction were determined as chlorojanerin and 13-acetyl solstitialin A which were elucidated by HR-ESI and (1)H, (13)C and 2D NMR spectroscopic techniques.

MeSH terms

  • Animals
  • Anti-Ulcer Agents / isolation & purification*
  • Biological Assay / methods
  • Centaurea*
  • Chemical Fractionation / methods*
  • Female
  • Flowers
  • Lactones / isolation & purification*
  • Lactones / therapeutic use
  • Male
  • Plant Extracts / isolation & purification
  • Plant Extracts / therapeutic use
  • Rats
  • Rats, Sprague-Dawley
  • Sesquiterpenes / isolation & purification*
  • Sesquiterpenes / therapeutic use
  • Stomach Ulcer / drug therapy

Substances

  • Anti-Ulcer Agents
  • Lactones
  • Plant Extracts
  • Sesquiterpenes