Culture-independent analysis of vaginal microflora: the unrecognized association of Atopobium vaginae with bacterial vaginosis

Am J Obstet Gynecol. 2004 Oct;191(4):1130-2. doi: 10.1016/j.ajog.2004.04.013.


Although the pathogenesis of bacterial vaginosis remains elusive, a few microorganisms, such as Gardnerella vaginalis, are considered markers on Gram stain or culture. Culture-independent analysis of vaginal microflora using 16S rDNA cloning and sequencing of total bacterial communities reveals the gram-positive Atopobium vaginae as a predominant species in disturbed vaginal flora.

MeSH terms

  • Actinobacteria / isolation & purification*
  • Adult
  • Bifidobacterium / isolation & purification
  • DNA, Ribosomal / isolation & purification
  • Female
  • Fusobacteria / isolation & purification
  • Humans
  • Leptotrichia / isolation & purification
  • Middle Aged
  • Polymorphism, Restriction Fragment Length
  • Vagina / microbiology*
  • Vaginosis, Bacterial / microbiology*


  • DNA, Ribosomal