Protein translocation across the peroxisomal membrane

Cell Biochem Biophys. 2004;41(3):451-68. doi: 10.1385/CBB:41:3:451.


Peroxisomal matrix proteins are synthesized on free cytosolic ribosomes and posttranslationally imported into the organelle. Translocation of these newly synthesized proteins across the peroxisomal membrane requires the concerted action of many different proteins, the majority of which were already identified. However, not much is known regarding the mechanism of protein translocation across this membrane system. Here, we discuss recent mechanistic and structural data. These results point to a model in which proteins en route to the peroxisomal matrix are translocated across the organelle membrane by their own receptor in a process that occurs through a large membrane protein assembly.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Biophysics / methods*
  • Cytoplasm / metabolism
  • Intracellular Membranes / metabolism
  • Membrane Proteins / chemistry*
  • Membrane Transport Proteins / chemistry
  • Models, Biological
  • Organelles
  • Peroxisomal Targeting Signal 2 Receptor
  • Peroxisome-Targeting Signal 1 Receptor
  • Peroxisomes / metabolism*
  • Phenotype
  • Pichia / metabolism
  • Protein Binding
  • Protein Transport*
  • Receptors, Cytoplasmic and Nuclear / chemistry
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / chemistry


  • Membrane Proteins
  • Membrane Transport Proteins
  • PEX5 protein, S cerevisiae
  • PEX7 protein, S cerevisiae
  • Peroxisomal Targeting Signal 2 Receptor
  • Peroxisome-Targeting Signal 1 Receptor
  • Receptors, Cytoplasmic and Nuclear
  • Saccharomyces cerevisiae Proteins