Herpesvirus infection of ICAM-1-deficient mice

Curr Eye Res. 2004 Aug-Sep;29(2-3):201-8. doi: 10.1080/02713680490504650.


Purpose: To determine the effect of ICAM-1 deficiency on viral infection of the cornea.

Materials and methods: Wild-type and intercellular adhesion molecule 1 (ICAM-1)-deficient mice were infected with the RE strain of herpes simplex virus type 1 (HSV-1). Corneal swabs and trigeminal ganglia were obtained and analyzed for infectious virus. Corneas and trigeminal ganglia were evaluated for signs of inflammation by immunohistochemical staining and for interferon-gamma (IFN-gamma)-producing cells by enzyme-linked immunospot assay (ELISPOT). Serum anti-HSV-1 antibody titers were determined by enzyme-linked immunosorbent assay (ELISA).

Results: Viral titers in corneal swabs from the wild-type and ICAM-1-deficient mice were not significantly different during the 21-day study. Infectious virus was present in the trigeminal ganglia of wild-type and ICAM-1-deficient mice through day 6 after infection. Serum anti-HSV-1 antibody titers were significantly higher in wild-type mice 6 days after infection, compared with ICAM-1-deficient mice; by day 8 and thereafter, however, antibody titers were not significantly different. Production of interferon gamma was greater in trigeminal ganglion cells from wild-type mice stimulated with interleukin 12 and interleukin 18 on days 4, 6, and 8 after infection compared with cells from ICAM-1-deficient mice. Histopathologic analysis of corneal and ganglion sections from wild-type and ICAM-1-deficient mice showed no significant differences in the time-course of appearance or the intensity of the inflammatory infiltrate. Immunohistochemical staining for CD3(+) T-lymphocytes and CD11b(+) neutrophils and macrophages demonstrated equivalent numbers of these cells in the corneas and trigeminal ganglia of wild-type and ICAM-1-deficient mice.

Conclusions: The results of these experiments indicate that ICAM-1 deficiency has only a modest effect on viral infection of the cornea and the development of an acquired immune response.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Viral / blood
  • Enzyme-Linked Immunosorbent Assay
  • Epithelium, Corneal / pathology
  • Epithelium, Corneal / virology
  • Female
  • Herpesvirus 1, Human* / immunology
  • Herpesvirus 1, Human* / isolation & purification
  • Immunohistochemistry / methods
  • Intercellular Adhesion Molecule-1 / genetics
  • Intercellular Adhesion Molecule-1 / metabolism*
  • Interferon-gamma / biosynthesis
  • Keratitis, Herpetic / etiology*
  • Keratitis, Herpetic / immunology
  • Keratitis, Herpetic / pathology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout / genetics
  • Staining and Labeling
  • Trigeminal Ganglion / metabolism
  • Trigeminal Ganglion / pathology
  • Trigeminal Ganglion / virology


  • Antibodies, Viral
  • Intercellular Adhesion Molecule-1
  • Interferon-gamma