Increased hypothalamic neuropeptide Y messenger RNA levels in two rat models of diabetes

Diabet Med. Jan-Feb 1992;9(1):76-80. doi: 10.1111/j.1464-5491.1992.tb01719.x.


Elevated levels of immunoreactive hypothalamic neuropeptide Y have recently been reported both in streptozotocin-induced diabetic rats and in the spontaneously diabetic BB rat. We have measured the levels of neuropeptide Y encoding messenger ribonucleic acid (mRNA) in both of these rat models to determine whether an increase in neuropeptide Y gene expression is a contributory factor to the increases in hypothalamic neuropeptide Y immunoreactive peptide content. In the hypothalami of both the spontaneously diabetic BB/E and the streptozotocin-diabetic animals, neuropeptide Y mRNA showed significant elevations (to 204 +/- 13% (+/- SE) and 387 +/- 48% of control values, respectively, p less than 0.01 for both). Our results demonstrate that two models of insulin-deficient diabetes in the rat are associated with increased hypothalamic neuropeptide Y mRNA. Taken with the known effects of neuropeptide Y on food intake these results suggest that increased neuropeptide Y synthesis in the hypothalamus may be related to the hyperphagia seen in the diabetic condition.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Glucose / metabolism
  • Blotting, Northern
  • DNA Probes
  • Diabetes Mellitus, Experimental / physiopathology*
  • Diabetes Mellitus, Type 1 / physiopathology*
  • Hypothalamus / physiopathology*
  • Insulin / blood
  • Male
  • Neuropeptide Y / genetics*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism*
  • Rats
  • Rats, Inbred BB
  • Rats, Inbred Strains


  • Blood Glucose
  • DNA Probes
  • Insulin
  • Neuropeptide Y
  • RNA, Messenger