Intein-mediated purification of a recombinantly expressed peptide

Chem Commun (Camb). 2004 Nov 7;(21):2412-3. doi: 10.1039/b407924h. Epub 2004 Sep 23.

Abstract

A 26 amino acid peptide has successfully been purified via recombinant expression as an intein fusion protein accompanied by cleavage without the need for any exogenous proteases or cofactors, thus offering a practical, inexpensive approach to produce isotopically labelled peptides.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Hydrogen-Ion Concentration
  • Inteins*
  • Peptides / chemistry*
  • Peptides / genetics
  • Peptides / isolation & purification*
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / isolation & purification
  • Temperature

Substances

  • Peptides
  • Recombinant Fusion Proteins