Sequence of the vanC gene of Enterococcus gallinarum BM4174 encoding a D-alanine:D-alanine ligase-related protein necessary for vancomycin resistance

Gene. 1992 Mar 1;112(1):53-8. doi: 10.1016/0378-1119(92)90302-6.


The amplification product obtained with DNA from vancomycin-resistant (VmR) Enterococcus gallinarum BM4174 and a pair of degenerate oligodeoxyribonucleotides that correspond to conserved amino acid (aa) motifs in Escherichia coli D-alanine (D-Ala):D-Ala ligases and in En. faecium VmR protein (VanA) was used as a probe to clone the vanC gene of that strain. The vanC product, with a calculated Mr of 37,504, exhibits 29 to 38% aa identity with VanA and E. coli ligases. Insertional inactivation of vanC led to Vm sensitivity of BM4174 suggesting that the gene may encode a D-Ala:D-Ala ligase of altered specificity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Blotting, Southern
  • DNA Mutational Analysis
  • Drug Resistance / genetics
  • Enterococcus / drug effects
  • Enterococcus / genetics*
  • Genes, Bacterial
  • Molecular Sequence Data
  • Peptide Synthases / chemistry
  • Peptide Synthases / genetics*
  • Vancomycin / pharmacology*


  • Bacterial Proteins
  • Vancomycin
  • Peptide Synthases
  • D-alanylalanine synthetase

Associated data

  • GENBANK/M75132
  • GENBANK/M76680
  • GENBANK/M76682
  • GENBANK/M79307
  • GENBANK/M79308
  • GENBANK/M79309
  • GENBANK/M79310
  • GENBANK/X13839
  • GENBANK/X60732
  • GENBANK/X60733