Molecular weight determination of hypromellose acetate succinate (HPMCAS) using size exclusion chromatography with a multi-angle laser light scattering detector

Chem Pharm Bull (Tokyo). 2004 Nov;52(11):1391-3. doi: 10.1248/cpb.52.1391.

Abstract

The molecular weight of hypromellose acetate succinate (HPMCAS), a polymer used for enteric coating, was determined by means of size exclusion chromatography with a multi-angle laser light scattering detector. The weight-average molecular weight (Mw) of several lots and grades ranged approximately from 17000 to 20000, and the number-average molecular weight (Mn) was around 13000. The inter-day precision of measurement, in terms of the coefficient of variation, was less than 5%.

MeSH terms

  • Chromatography, Gel / methods*
  • Lasers
  • Light
  • Methylcellulose / analogs & derivatives*
  • Methylcellulose / analysis*
  • Molecular Weight
  • Scattering, Radiation

Substances

  • hydroxypropylmethylcellulose acetate succinate
  • Methylcellulose