Distinct T cell dynamics in lymph nodes during the induction of tolerance and immunity

Nat Immunol. 2004 Dec;5(12):1235-42. doi: 10.1038/ni1134. Epub 2004 Oct 31.


Induction of immunity and peripheral tolerance requires contacts between antigen-bearing dendritic cells (DCs) and cognate T cells. Using real-time two-photon microscopy, we have analyzed the dynamics of CD8(+) T cells in lymph nodes during the induction of antigen-specific immunity or tolerance. At 15-20 h after the induction of immunity, T cells stopped moving and established prolonged interactions with DCs. In tolerogenic conditions, despite effective initial T cell activation and proliferation, naive T cells remained motile and established serial brief contacts with multiple DCs. Thus, stable DC-T cell interactions occur during the induction of priming, whereas brief contacts may contribute to the induction of T cell tolerance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Communication
  • Cell Differentiation
  • Cell Movement*
  • Dendritic Cells / cytology
  • Dendritic Cells / immunology
  • Immune Tolerance / immunology*
  • Lymph Nodes / cytology*
  • Lymph Nodes / immunology*
  • T-Lymphocytes / classification
  • T-Lymphocytes / cytology*
  • T-Lymphocytes / immunology*
  • Time Factors