Structural investigation of a novel rhamnoglucogalactan isolated from the fruiting bodies of the fungus Hericium erinaceus

Carbohydr Res. 2004 Nov 15;339(16):2667-71. doi: 10.1016/j.carres.2004.07.027.

Abstract

A new heteropolysaccharide (HEP-1) was isolated from the fruiting bodies of Hericium erinaceus. It was estimated to have a molecular weight of 1.8x10(4) da and showed [alpha](D)(20) +129 (c 0.295, H(2)O). HEP-1 is composed of rhamnose, galactose, and glucose in the ratio of 1.19:3.81:1.00. Its structural features were investigated using composition analysis, methylation analysis, partial hydrolysis, and IR and NMR spectroscopy. The results showed that HEP-1 has a (1-->6)-linked alpha-d-galactopyranosyl backbone with branches that are composed of rhamnose and glucose attached to O-2.

MeSH terms

  • Basidiomycota / chemistry
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Fruiting Bodies, Fungal / chemistry*
  • Galactose / analysis
  • Gas Chromatography-Mass Spectrometry
  • Glucose / analysis
  • Magnetic Resonance Spectroscopy
  • Polysaccharides / chemistry*
  • Polysaccharides / isolation & purification
  • Rhamnose / analysis

Substances

  • Polysaccharides
  • Glucose
  • Rhamnose
  • Galactose