Green tea extract attenuates cyclosporine A-induced oxidative stress in rats

Pharmacol Res. 2005 Jan;51(1):51-7. doi: 10.1016/j.phrs.2004.04.007.

Abstract

Cyclosporine A (CsA) nephrotoxicity underweighs the therapeutic benefits of such a powerful immunosuppressant. Whether oxidative stress plays a role in such toxicity is not well delineated. We investigated the potential of green tea extract (GTE) to attenuate CsA-induced renal dysfunction in rats. Three main groups of Sprague-Dawley rats were used: CsA, GTE, and GTE plus CsA-receiving animals. Corresponding control groups were also used. CsA was administered in a dose of 20mg kg(-1) day(-1), i.p., for 21 days. In the GTE/CsA groups, the rats received different concentrations of GTE (0.5, 1.0 and 1.5%), as their sole source of drinking water, 4 days before and 21 days concurrently with CsA. The GTE group was treated with 1.5% concentration of GTE only for 25 days. A concomitant administration of GTE, to CsA receiving rats, markedly prevented the generation of thiobarbituric acid-reacting substances (TBARS) and significantly attenuated CsA-induced renal dysfunction as assessed by estimating serum creatinine, blood urea nitrogen, uric acid and urinary excretion of glucose. A considerable improvement in terms of reduced glutathione content and activity of antioxidant enzymes in the kidney homogenate of the GTE/CsA-receiving rats was observed. The activity of lysosomal enzymes, N-acetyl-beta-glucosaminidase, beta-glucuronidase and acid phosphatase was significantly inhibited following GTE co-administration. Our data prove the role of oxidative stress in the pathogenesis of CsA-induced kidney dysfunction. Supplementation of GTE could be useful in reducing CsA nephrotoxicity in rats. However, clinical studies are warranted to investigate such an effect in human subjects.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Cyclosporine / antagonists & inhibitors
  • Cyclosporine / pharmacology*
  • Dose-Response Relationship, Drug
  • Male
  • Oxidative Stress / drug effects*
  • Oxidative Stress / physiology
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Tea*

Substances

  • Plant Extracts
  • Tea
  • Cyclosporine