Rescue of Ebola virus from cDNA using heterologous support proteins

Virus Res. 2004 Nov;106(1):43-50. doi: 10.1016/j.virusres.2004.06.002.


Using the infectious clone for Zaire ebolavirus, the functional specificity of viral proteins of the ribonucleoprotein complex in transcription/replication was investigated by substituting them with heterologous proteins derived from closely (Reston ebolavirus) and distantly related filoviruses (Marburgvirus). The data clearly demonstrated that transcription/replication are neither strictly species-specific nor genus-specific. Protein interactions between the nucleoprotein NP and the virion protein VP35 and the polymerase L and VP35 seemed to be the most critical steps. In contrast to previous data, viral proteins were able to target heterologous filovirus RNA. Together these results indicated that protein-protein interactions are more critical than protein-RNA interactions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Heterophile
  • Chlorocebus aethiops
  • DNA, Complementary
  • Ebolavirus / genetics*
  • Ebolavirus / physiology
  • Filoviridae / classification
  • Filoviridae / genetics*
  • Filoviridae / physiology
  • Nucleocapsid Proteins / genetics
  • Nucleocapsid Proteins / metabolism*
  • Ribonucleoproteins / chemistry
  • Ribonucleoproteins / genetics
  • Ribonucleoproteins / metabolism*
  • Vero Cells
  • Viral Proteins / genetics


  • Antigens, Heterophile
  • DNA, Complementary
  • Nucleocapsid Proteins
  • Ribonucleoproteins
  • Viral Proteins