Rapid detection of Panton-Valentine leukocidin from clinical isolates of Staphylococcus aureus strains by real-time PCR

FEMS Microbiol Lett. 2004 Nov 15;240(2):225-8. doi: 10.1016/j.femsle.2004.09.031.

Abstract

To allow rapid identification of Panton-Valentine leukocidin (PVL)-producing Staphylococcus aureus strains, a real-time PCR assay for detection of PVL was developed. This assay is convenient, since it can be applied directly on bacterial suspensions and does not require previous DNA purification. Furthermore, the assay was found to be highly reproducible, robust and specific, since positive results were generated exclusively with PVL-positive S. aureus strains, and neither with PVL-negative strains nor staphylococci other than S. aureus.

Publication types

  • Evaluation Study

MeSH terms

  • Bacterial Toxins
  • Exotoxins
  • Humans
  • Leukocidins / genetics
  • Leukocidins / isolation & purification*
  • Polymerase Chain Reaction*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Staphylococcal Infections / microbiology*
  • Staphylococcus aureus / genetics*
  • Staphylococcus aureus / isolation & purification

Substances

  • Bacterial Toxins
  • Exotoxins
  • Leukocidins
  • Panton-Valentine leukocidin