Confocal microscopy of the in-situ crystalline lens

J Microsc. 1992 Jan;165(Pt 1):159-67. doi: 10.1111/j.1365-2818.1992.tb04313.x.


The fine structure of the in-situ rabbit crystalline ocular lens from the ex-vivo rabbit eye was observed with a confocal scanning laser microscope in the scattered light mode. The images were observed through the full thickness of the cornea and aqueous humour to a depth of 50 microns in the anterior ocular lens. The following structures were observed from optical sections of the ocular lens: two concentric regions of the lens capsule, epithelial cells, lens sutures, and surface and interior regions of individual lenticular fibres. The observed lateral resolution of the microscope objective was degraded by imaging across thick (millimetre) structures. This study shows the feasibility of obtaining high-contrast images of transparent objects across 1.7 mm of ocular tissue (cornea and aqueous humour) using confocal light microscopy.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Epithelial Cells
  • Epithelium / ultrastructure
  • Lasers
  • Lens, Crystalline / cytology
  • Lens, Crystalline / ultrastructure*
  • Male
  • Microscopy / methods*
  • Rabbits