Synchronization in hybrid neuronal networks of the hippocampal formation

J Neurophysiol. 2005 Mar;93(3):1197-208. doi: 10.1152/jn.00982.2004. Epub 2004 Nov 3.

Abstract

Understanding the mechanistic bases of neuronal synchronization is a current challenge in quantitative neuroscience. We studied this problem in two putative cellular pacemakers of the mammalian hippocampal theta rhythm: glutamatergic stellate cells (SCs) of the medial entorhinal cortex and GABAergic oriens-lacunosum-molecular (O-LM) interneurons of hippocampal region CA1. We used two experimental methods. First, we measured changes in spike timing induced by artificial synaptic inputs applied to individual neurons. We then measured responses of free-running hybrid neuronal networks, consisting of biological neurons coupled (via dynamic clamp) to biological or virtual counterparts. Results from the single-cell experiments predicted network behaviors well and are compatible with previous model-based predictions of how specific membrane mechanisms give rise to empirically measured synchronization behavior. Both cell types phase lock stably when connected via homogeneous excitatory-excitatory (E-E) or inhibitory-inhibitory (I-I) connections. Phase-locked firing is consistently synchronous for either cell type with E-E connections and nearly anti-synchronous with I-I connections. With heterogeneous connections (e.g., excitatory-inhibitory, as might be expected if members of a given population had heterogeneous connections involving intermediate interneurons), networks often settled into phase locking that was either stable or unstable, depending on the order of firing of the two cells in the hybrid network. Our results imply that excitatory SCs, but not inhibitory O-LM interneurons, are capable of synchronizing in phase via monosynaptic mutual connections of the biologically appropriate polarity. Results are largely independent of synaptic strength and synaptic kinetics, implying that our conclusions are robust and largely unaffected by synaptic plasticity.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Electric Stimulation / methods
  • Excitatory Postsynaptic Potentials / physiology
  • Excitatory Postsynaptic Potentials / radiation effects
  • Glutamic Acid / metabolism
  • Hippocampus / cytology
  • Hippocampus / physiology*
  • Nerve Net / physiology*
  • Neural Inhibition / physiology
  • Neural Networks, Computer*
  • Neurons / classification
  • Neurons / physiology*
  • Patch-Clamp Techniques
  • Rats
  • Reaction Time
  • Synapses / physiology
  • Synaptic Transmission / physiology*
  • Synaptic Transmission / radiation effects
  • Time Factors
  • gamma-Aminobutyric Acid / metabolism

Substances

  • Glutamic Acid
  • gamma-Aminobutyric Acid