Genomic organization of the 8p11-p12 amplicon in three breast cancer cell lines

Cancer Genet Cytogenet. 2004 Nov;155(1):57-62. doi: 10.1016/j.cancergencyto.2004.03.013.

Abstract

Amplification of chromosomal regions leads to an increase of DNA copy number and expression of oncogenes in human breast cancer (HBC). Amplification of the 8p11-p12 region occurs in 10-15% of primary, uncultured HBCs. In our panel of 11 breast cancer cells, three cell lines, SUM-44, SUM-52, and SUM-225, have overlapping amplicons in the 8p11-p12 region. To characterize genome structure of the amplified regions, we performed fluorescence in situ hybridization using 8p11-p12 BAC clones in the 3 cell lines. The results revealed that the 8p11-p12 amplicon has a highly complex structure and that FGFR1 is not in the common core-amplified domain in 3 breast cancer cell lines with the amplicon. These 3 cell lines provide good models for genetic and functional studies of candidate oncogenes of the 8p11-p12 region.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Breast Neoplasms / genetics*
  • Cell Line, Tumor
  • Chromosomes, Human, Pair 8*
  • Gene Amplification
  • Humans
  • In Situ Hybridization, Fluorescence
  • Models, Genetic
  • Nucleic Acid Hybridization