Protein A gene expression is regulated by DNA supercoiling which is modified by the ArlS-ArlR two-component system of Staphylococcus aureus

Microbiology. 2004 Nov;150(Pt 11):3807-3819. doi: 10.1099/mic.0.27194-0.

Abstract

Bacterial pathogens such as Staphylococcus aureus undergo major physiological changes when they infect their hosts, requiring the coordinated regulation of gene expression in response to the stresses encountered. Several environmental factors modify the expression of S. aureus virulence genes. This report shows that the expression of spa (virulence gene encoding the cell-wall-associated protein A) is down-regulated by high osmolarity (1 M NaCl, 1 M KCl or 1 M sucrose) in the wild-type strain and upregulated by novobiocin (a DNA gyrase inhibitor that relaxes DNA). A gyrB142 allele corresponding to a double mutation in the B subunit of DNA gyrase relaxed DNA and consequently induced spa expression, confirming that spa expression is regulated by DNA topology. Furthermore, in the presence of novobiocin plus 1 M NaCl, a good correlation was observed between DNA supercoiling and spa expression. The ArlS-ArlR two-component system is involved in the expression of virulence genes such as spa. Presence of an arlRS deletion decreased the effect of DNA supercoiling modulators on spa expression, suggesting that active Arl proteins are necessary for the full effect of DNA gyrase inhibitors and high osmolarity on spa expression. Indeed, evidence is provided for a relationship between the arlRS deletion and topological changes in plasmid DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Physiological
  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • DNA Gyrase / genetics
  • DNA Gyrase / metabolism
  • DNA, Bacterial / chemistry
  • DNA, Superhelical / genetics
  • DNA, Superhelical / metabolism*
  • Enzyme Inhibitors / pharmacology
  • Gene Deletion
  • Gene Expression Regulation, Bacterial*
  • Novobiocin / pharmacology
  • Nucleic Acid Conformation
  • Osmotic Pressure
  • Protein Kinases / genetics*
  • Protein Kinases / metabolism
  • Signal Transduction
  • Staphylococcal Protein A / biosynthesis*
  • Staphylococcal Protein A / genetics
  • Staphylococcus aureus / genetics*
  • Staphylococcus aureus / metabolism*
  • Virulence Factors / biosynthesis
  • Virulence Factors / genetics

Substances

  • ArlR protein, Staphylococcus aureus
  • Bacterial Proteins
  • DNA, Bacterial
  • DNA, Superhelical
  • Enzyme Inhibitors
  • Staphylococcal Protein A
  • Virulence Factors
  • Novobiocin
  • Protein Kinases
  • ArlS protein, Staphylococcus aureus
  • DNA Gyrase