Identification of a novel nephropathogenic infectious bronchitis virus in Israel

Avian Dis. 2004 Sep;48(3):635-41. doi: 10.1637/7107.


A novel infectious bronchitis variant, designated as IS/885/00, associated with nephritis, was isolated from outbreaks in 23 broiler farms in Israel. The virus was first identified by reverse transcriptase-polymerase chain reaction and showed a distinct restriction fragment length polymorphism pattern from previously described Israeli isolates. Sequence analysis of the S1 gene and the deduced amino acid sequence revealed 97.2% protein similarity to genotype IS/ 720/99 and 71.6% similarity to the vaccine strain H120, the only strain permitted for use in this country. A database search in GenBank revealed a closely related isolate from Egypt, Egypt/Beni-Seug/01, with 96.6% similarity. Other published nephropathogenic infectious bronchitis virus strains/isolates shared less than 77% similarity with IS/885/00. A vaccine protection test in specific-pathogen-free chicks indicated 91% protection to the trachea and only 25% protection to the kidneys in vaccinated birds challenged with IS/885/00.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Capsid Proteins / genetics
  • Chickens / immunology
  • Chickens / virology*
  • Cluster Analysis
  • DNA Primers
  • Enzyme-Linked Immunosorbent Assay
  • Infectious bronchitis virus / genetics*
  • Infectious bronchitis virus / immunology*
  • Israel
  • Molecular Sequence Data
  • Phylogeny
  • Polymorphism, Restriction Fragment Length
  • Poultry Diseases / immunology
  • Poultry Diseases / virology*
  • Reverse Transcriptase Polymerase Chain Reaction / veterinary
  • Sequence Analysis, DNA
  • Sequence Homology
  • Trachea / virology
  • Viral Vaccines / immunology*


  • Capsid Proteins
  • DNA Primers
  • Viral Vaccines
  • sigma 1 protein, reovirus