A new putative cyclic nucleotide-gated channel gene, cng-3, is critical for thermotolerance in Caenorhabditis elegans

Biochem Biophys Res Commun. 2004 Dec 10;325(2):525-31. doi: 10.1016/j.bbrc.2004.10.060.


Cyclic nucleotide-gated (CNG) channels encoded by tax-4 and tax-2 genes are required for chemo- and thermo-sensation in Caenorhabditis elegans. Here we report the identification and the characterization of cng-3, a new CNG channel gene, found in C. elegans. CNG-3 contains six putative transmembrane regions and a cyclic nucleotide-binding domain that show high homology with CNG channels of higher animals as well as TAX-4. The expression of cng-3 is detected from early stages in worm development and restricted in five sensory neurons of amphid including AFD neuron. While a cng-3 null mutant displays normal chemotaxis to volatile odorants, the mutant worms exhibit impaired thermal tolerance. These results indicate that CNG-3, a new member of CNG channel subunits, may play a critical role in sensation or response of thermal stress in C. elegans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohols / chemistry
  • Alcohols / pharmacology
  • Aldehydes / chemistry
  • Aldehydes / pharmacology
  • Amino Acid Sequence
  • Animals
  • Caenorhabditis elegans / chemistry
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / physiology*
  • Chemotaxis / genetics
  • Cyclic Nucleotide-Gated Cation Channels
  • Gene Deletion
  • Gene Expression
  • Genome Components / genetics
  • Heat-Shock Response / physiology*
  • Ion Channels / chemistry
  • Ion Channels / genetics*
  • Ion Channels / physiology*
  • Molecular Sequence Data
  • Phylogeny
  • Protein Subunits
  • Pyrazines / pharmacology
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Sequence Alignment
  • Smell / genetics
  • Thiazoles / pharmacology


  • Alcohols
  • Aldehydes
  • Cyclic Nucleotide-Gated Cation Channels
  • Ion Channels
  • Protein Subunits
  • Pyrazines
  • Recombinant Proteins
  • Thiazoles
  • 2,4,5-trimethylthiazole