Abstract
Edible vaccines have been generated from both transgenic plants as well as from plant viral vectors. Here, we have taken the best attributions of both systems and designed a minimalized version of the bean yellow dwarf geminivirus (BeYDV)-based replicon consisting of the cis-acting elements required for BeYDV replication as a means to express foreign genes in a plant cell line. Replication can be switched on at high levels upon expression of the BeYDV Rep protein, and gene expression enhanced enormously. Construction of an expression cassette encoding a synthetic vaccine gene and analysis of expression levels of a vaccine protein in a plant cell line system are described.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Antigens, Bacterial / biosynthesis*
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Antigens, Bacterial / genetics
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Blotting, Southern
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Cell Line
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Cells, Cultured
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Enterotoxins / biosynthesis*
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Enterotoxins / genetics
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Enzyme-Linked Immunosorbent Assay
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Geminiviridae / genetics*
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Gene Expression Regulation
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Genes, Reporter
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Green Fluorescent Proteins / biosynthesis
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Green Fluorescent Proteins / genetics
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Nicotiana / cytology
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Nicotiana / genetics
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Nicotiana / metabolism*
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Plants, Genetically Modified / cytology
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Plants, Genetically Modified / metabolism
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Replicon
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Vaccines, Edible / genetics
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Virus Replication
Substances
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Antigens, Bacterial
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Enterotoxins
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Vaccines, Edible
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Green Fluorescent Proteins
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enterotoxin B, staphylococcal