The effects of temperature reduction on gene expression and oxidative stress in skeletal muscle from adult zebrafish

Comp Biochem Physiol C Toxicol Pharmacol. 2004 Jul;138(3):363-73. doi: 10.1016/j.cca.2004.08.014.

Abstract

Longevity is inversely proportional to ambient temperature in ectothermic organisms such as fish. However, the mechanism by which reducing temperature over a physiological range increases life span is not known and available data are derived primarily from invertebrates. With a rodent-like longevity and abundant biological resources, the zebrafish is an ideal vertebrate ectothermic model in which to investigate this phenomenon. As an initial approach, the effects of a year-long 10 degrees C reduction in water temperature on global gene expression in tail skeletal muscle from adult zebrafish were determined using an oligonucleotide microarray representing 15,512 genes. Expression levels for approximately 600 genes were up-regulated by 1.7-fold or greater by the reduction in temperature, while a similar number of transcripts were down regulated by more than 1.7-fold. Using gene ontology (GO) classifications for molecular function, two functional groups, "oxygen and reactive oxygen species metabolism" and "response to oxidative stress," were found to be overrepresented among up-regulated genes. Transcripts levels for the genes in these two categories were increased by temperature reduction (TR). However, temperature reduction did not suppress lipid peroxidation potential, protein carbonyl content, or 8-oxoguanine level. Additional studies will be required to further delineate the role of altered gene expression and oxidative stress on the longevity-promoting effects of temperature reduction.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aging / physiology*
  • Animals
  • Down-Regulation / genetics
  • Feeding Behavior
  • Gene Expression Profiling / standards
  • Gene Expression Regulation*
  • Muscle, Skeletal / metabolism*
  • Oligonucleotide Array Sequence Analysis / standards
  • Oxidative Stress*
  • Quality Control
  • Temperature*
  • Zebrafish / genetics*
  • Zebrafish / growth & development
  • Zebrafish Proteins / genetics

Substances

  • Zebrafish Proteins