Localization of zinc transporter-3 (ZnT-3) in mouse retina

Vision Res. 2004 Dec;44(28):3317-21. doi: 10.1016/j.visres.2004.07.038.

Abstract

Studies of the central nervous system have localized the zinc-transporter-3 (ZnT-3) protein to synaptic vesicles containing glutamate and zinc. We have examined the distribution of the ZnT-3 protein in the light-adapted mouse retina using immunohistochemical techniques. Light microscopic analysis of 15-30-microm retinal sections revealed a rich band of ZnT-3 protein in the region of the outer limiting membrane and photoreceptor inner segments. ZnT-3 reactivity was also present in the outer plexiform, inner nuclear, inner plexiform, and ganglion cell layers. The outer nuclear layer and photoreceptor outer segments did not exhibit ZnT-3 immunoreactivity. In the light-adapted murine retina, ZnT-3 appears localized in regions which have been found reactive for ionic zinc.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptation, Ocular / physiology
  • Animals
  • Carrier Proteins / analysis*
  • Cation Transport Proteins
  • Immunoenzyme Techniques
  • Male
  • Membrane Proteins / analysis*
  • Membrane Transport Proteins
  • Mice
  • Mice, Inbred C57BL
  • Retina / chemistry*
  • Zinc / metabolism

Substances

  • Carrier Proteins
  • Cation Transport Proteins
  • Membrane Proteins
  • Membrane Transport Proteins
  • Slc30a3 protein, mouse
  • Zinc