Sputum and bronchial submucosal IL-13 expression in asthma and eosinophilic bronchitis

J Allergy Clin Immunol. 2004 Nov;114(5):1106-9. doi: 10.1016/j.jaci.2004.08.032.


Background: Nonasthmatic eosinophilic bronchitis is a condition characterized by the presence of eosinophilic airway inflammation in the absence of airflow obstruction or airway hyperresponsiveness. In asthma, the T H 2-type cytokine IL-13 has been implicated in the development of airway inflammation and hyperresponsiveness. Whether the expression of IL-13 is different between these 2 conditions is unknown.

Objective: We sought to investigate whether IL-13 expression is increased in asthma compared with eosinophilic bronchitis.

Methods: Sputum samples from subjects with mild asthma (n = 30) and eosinophilic bronchitis (n = 15) and normal controls (n = 16) were dialyzed, and IL-13 concentration was measured by ELISA. In a subgroup of these patients, IL-13 protein expression in bronchial biopsies was assessed by immunohistochemistry.

Results: The concentration of sputum IL-13 was higher in patients with mild asthma than in normal controls ( P = .03) and in patients with eosinophilic bronchitis ( P = .03). The median (interquartile range) number of IL-13 + cells/mm 2 submucosa was significantly higher in asthma 4 (8) than eosinophilic bronchitis 1.7 (1.9) and normal controls 0.5 (1.1; P = .004). Eighty-three percent of the cells expressing IL-13 in the submucosa were eosinophils, and 8% were mast cells. The median (interquartile range) proportion of eosinophils that expressed IL-13 was higher in the subjects with asthma, 16 (10)%, than those with eosinophilic bronchitis, 7 (3)% ( P = .02).

Conclusion: The increased expression of IL-13 in asthma compared with eosinophilic bronchitis supports the concept that IL-13 may play a critical role in the pathophysiology of asthma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Asthma / immunology*
  • Bronchi / chemistry*
  • Bronchitis / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Eosinophilia / immunology*
  • Female
  • Humans
  • Interleukin-13 / analysis*
  • Male
  • Middle Aged
  • Respiratory Mucosa / chemistry
  • Sputum / chemistry*


  • Interleukin-13