Expression of kinin B1 receptors in the spinal cord of streptozotocin-diabetic rat

Neuroreport. 2004 Nov 15;15(16):2463-6. doi: 10.1097/00001756-200411150-00006.

Abstract

Previous studies have reported cardiovascular and nociceptive responses after intrathecal injection of kinin B1 receptor (B1R) agonists in the model of streptozotocin (STZ)-diabetic rat (diabetic). The aim of this study was to measure the early up-regulation of B1R binding sites and mRNA in the thoracic spinal cord of diabetic and control rats. Data show significant increases of specific B1R binding sites in the dorsal horn of diabetic rats 2 days (+315%), 7 days (+303%) and 21 days (+181%) after STZ treatment. Levels of mRNA were significantly increased (+68%) at 2 and 7 days but not at 21 days. These data bring the first molecular evidence for an early up-regulation of B1R in the spinal cord of diabetic rat.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Glucose / metabolism
  • Body Weight / physiology
  • Bradykinin / analogs & derivatives*
  • Bradykinin / pharmacokinetics
  • Diabetes Mellitus, Experimental / metabolism*
  • Gene Expression Regulation / drug effects
  • In Situ Hybridization / methods
  • Iodine Isotopes / pharmacokinetics
  • Male
  • Protein Binding / drug effects
  • Protein Binding / physiology
  • RNA, Messenger / metabolism
  • Radiography / methods
  • Rats
  • Rats, Wistar
  • Receptor, Bradykinin B1 / genetics
  • Receptor, Bradykinin B1 / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Spinal Cord / metabolism*
  • Tetrahydroisoquinolines / pharmacokinetics
  • Time Factors

Substances

  • Blood Glucose
  • Iodine Isotopes
  • RNA, Messenger
  • Receptor, Bradykinin B1
  • Tetrahydroisoquinolines
  • HOE 140, desArg(10)-
  • Bradykinin