Genetically programmed B lymphocytes are highly efficient in inducing anti-virus protective immunity mediated by central memory CD8 T cells

Vaccine. 2004 Dec 16;23(5):699-708. doi: 10.1016/j.vaccine.2004.06.028.

Abstract

The hallmarks of specific T cell immunity include proliferative expansion, acquisition of effector function and memory T cell formation. Here, we used priming with B lymphocytes transgenic for the dominant epitope (NP366-374) of the influenza virus nucleoprotein, to study the characteristics of the CD8 T cell memory response in C57Bl/6 mice and elucidate which subset of CD8 T cells memory mediates protection from disease. We found that (i) the size of the memory CTL response is independent of the priming dose and is similar to that induced by the live virus, (ii) priming with a low dose (3 x 10(2)cells/inoculum) of transgenic B lymphocytes confers a protective memory CTL response, and (iii) protection from disease is mediated by central memory (T(CM)) CD8 T cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigen-Presenting Cells / immunology*
  • B-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / immunology*
  • Disease Models, Animal
  • Dose-Response Relationship, Immunologic
  • Epitopes / genetics
  • Epitopes / immunology
  • Immunologic Memory*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Orthomyxoviridae / genetics
  • Orthomyxoviridae / immunology*
  • Orthomyxoviridae Infections / immunology*
  • Peptide Fragments / genetics
  • Peptide Fragments / immunology
  • T-Lymphocyte Subsets / immunology*
  • Vaccines, Synthetic / genetics
  • Vaccines, Synthetic / immunology
  • Viral Core Proteins / genetics
  • Viral Core Proteins / immunology

Substances

  • Epitopes
  • Peptide Fragments
  • Vaccines, Synthetic
  • Viral Core Proteins
  • nucleoprotein (366-374), influenza virus