Is the mycobacteria-derived purified protein response in atopic asthmatic children different?

Int Arch Allergy Immunol. 2004 Nov;135(3):229-34. doi: 10.1159/000081308. Epub 2004 Oct 6.


Background: The response to mycobacteria-derived purified protein (PPD) is mediated primarily by T-helper-1 response and is expected to be inhibited in atopic diseases. The aim of this study was to investigate whether the PPD response is different in atopic asthmatic children.

Methods: 40 atopic asthmatic children (mean age 8.3 +/- 4.9 years) and 40 healthy age- and sex-matched children who had received bacillus Calmette-Guerin (BCG) vaccination were included in the study. Five PPD units were administered intradermally to all children and were interpreted after 48 h.

Results: There was no correlation between serum total IgE level and PPD induration (p=0.054). The PPD induration was not statistically different between the children who used inhaled corticosteroid and those who did not. Although the PPD positivity (induration > or =5 mm) rate was higher in atopic asthmatic children (50%) than in healthy children (32.5%), the difference was not found to be statistically significant. The PPD induration in atopic asthmatic children (7.41 +/- 5.58 mm) was found to be greater than the one in healthy children (5.21 +/- 3.39) (p < 0.039). The induration in atopic asthmatic children (5.21 +/- 3.77) and healthy children (4.43 +/- 2.32) did not show a difference in children who where vaccinated only once with BCG, but it was found to be statistically significantly greater in atopic asthmatic children (12.50 +/- 5.90) than healthy children (7.08 +/- 4.70) who were vaccinated with BCG twice (p <0.012). The proportion of having a PPD induration of > or =10 mm was found to be higher in atopic asthmatic children than in the healthy ones (32.5 vs. 12.5%) (p <0.032).

Conclusion: Our data showed that the PPD response was stronger in BCG-vaccinated atopic asthmatic children than in healthy BCG-vaccinated ones.

MeSH terms

  • Adolescent
  • Asthma / immunology*
  • Asthma / microbiology
  • BCG Vaccine / immunology*
  • Child
  • Child, Preschool
  • Female
  • Humans
  • Hypersensitivity, Delayed / immunology
  • Immunoglobulin E / blood*
  • Male
  • Mycobacterium / immunology
  • Prospective Studies
  • Statistics, Nonparametric
  • Tuberculin / immunology*
  • Turkey


  • BCG Vaccine
  • Tuberculin
  • Immunoglobulin E