Adapter protein 14-3-3 is required for a presynaptic form of LTP in the cerebellum

Nat Neurosci. 2004 Dec;7(12):1296-8. doi: 10.1038/nn1348. Epub 2004 Nov 14.

Abstract

Long-term potentiation (LTP) of granule cell-Purkinje cell synapses in the mouse cerebellum requires phosphorylation by protein kinase A of the active-zone protein RIM1alpha at Ser413. Here, we show that the adapter protein 14-3-3 readily binds phosphorylated Ser413 in RIM1alpha, and that presynaptic transfection with a dominant-negative 14-3-3eta mutant, or a RIM1alpha mutant with enhanced 14-3-3 binding, inhibits LTP. Thus, RIM1alpha phosphorylation triggers presynaptic LTP in part through recruitment of 14-3-3 to phospho-Ser413-RIM1alpha.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 14-3-3 Proteins / biosynthesis
  • 14-3-3 Proteins / genetics
  • 14-3-3 Proteins / physiology*
  • Animals
  • Cerebellum / cytology
  • Cerebellum / metabolism
  • Cerebellum / physiology*
  • Long-Term Potentiation / physiology*
  • Mice
  • Mutation
  • Neural Inhibition / genetics
  • Neural Inhibition / physiology
  • Presynaptic Terminals / physiology*

Substances

  • 14-3-3 Proteins