Brevibacterium celere sp. nov., isolated from degraded thallus of a brown alga

Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2107-2111. doi: 10.1099/ijs.0.02867-0.

Abstract

Two whitish yellow, Gram-positive, non-motile, aerobic bacteria were isolated from enrichment culture during degradation of the thallus of the brown alga Fucus evanescens. The bacteria studied were chemo-organotrophic, mesophilic and grew well on nutrient media containing up to 15 % (w/v) NaCl. The DNA G+C content was 61 mol%. The two isolates exhibited a conspecific DNA-DNA relatedness value of 98 %, indicating that they belong to the same species. A comparative analysis of 16S rRNA gene sequences revealed that strain KMM 3637(T) formed a distinct phyletic lineage in the genus Brevibacterium (family Brevibacteriaceae, class Actinobacteria) and showed the highest sequence similarity (about 97 %) to Brevibacterium casei. DNA-DNA hybridization experiments demonstrated 45 % binding with the DNA of B. casei DSM 20657(T). Physiological and chemotaxonomic characteristics (meso-diaminopimelic acid in the peptidoglycan, major cellular fatty acids 15 : 0ai and 17 : 0ai) of the bacteria studied were consistent with the genomic and phylogenetic data. On the basis of the results of this study, a novel species, Brevibacterium celere sp. nov., is proposed. The type strain is KMM 3637(T) (=DSM 15453(T)=ATCC BAA-809(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aerobiosis
  • Bacterial Typing Techniques
  • Base Composition
  • Brevibacterium / classification*
  • Brevibacterium / cytology
  • Brevibacterium / isolation & purification*
  • Brevibacterium / physiology
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / isolation & purification
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / isolation & purification
  • Diaminopimelic Acid / analysis
  • Diaminopimelic Acid / isolation & purification
  • Fatty Acids / analysis
  • Fatty Acids / isolation & purification
  • Fucus / microbiology*
  • Genes, rRNA
  • Gentian Violet
  • Molecular Sequence Data
  • Movement
  • Nucleic Acid Hybridization
  • Organic Chemicals / metabolism
  • Peptidoglycan / chemistry
  • Peptidoglycan / isolation & purification
  • Phenazines
  • Phylogeny
  • Pigments, Biological / biosynthesis
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Saline Solution, Hypertonic
  • Sequence Analysis, DNA
  • Temperature

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Gram's stain
  • Organic Chemicals
  • Peptidoglycan
  • Phenazines
  • Pigments, Biological
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Saline Solution, Hypertonic
  • Diaminopimelic Acid
  • Gentian Violet

Associated data

  • GENBANK/AY228463