Abstract
A strictly aerobic, non-motile, rod-shaped actinomycete, designated strain JC2056(T), was isolated from a sediment sample of getbol, the tidal flat of Korea. Results of 16S rRNA gene sequence analysis indicated that the isolate belonged to the genus Nocardioides, with the highest similarity being to Nocardioides luteus KCTC 9575(T) (95.7 %). The major menaquinone was MK-8(H(4)), and predominant cellular fatty acids were iso-16 : 0 and iso-16 : 1 H. The DNA G+C content was 70 mol%. Based on the morphological, physiological, biochemical and chemotaxonomical data presented in this study, strain JC2056(T) should be classified as a novel species, for which the name Nocardioides aestuarii sp. nov. is proposed; the type strain is JC2056(T) (=IMSNU 14029(T)=KCTC 9921(T)=JCM 12125(T)).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actinomycetales / classification*
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Actinomycetales / genetics
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Actinomycetales / isolation & purification*
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Actinomycetales / physiology
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Bacterial Typing Techniques
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Base Composition
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Cyanoacrylates / analysis
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DNA, Bacterial / chemistry
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DNA, Bacterial / isolation & purification
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / isolation & purification
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Fatty Acids / analysis
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Fatty Acids / isolation & purification
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Genes, rRNA
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Geologic Sediments / microbiology*
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Korea
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Molecular Sequence Data
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Phylogeny
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RNA, Bacterial / genetics
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
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Vitamin K 2 / analysis
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Vitamin K 2 / isolation & purification
Substances
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Cyanoacrylates
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DNA, Bacterial
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DNA, Ribosomal
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Fatty Acids
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RNA, Bacterial
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RNA, Ribosomal, 16S
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Vitamin K 2
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cyacrin
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vitamin MK 8