Type II isopentenyl diphosphate isomerase from Synechocystis sp. strain PCC 6803

J Bacteriol. 2004 Dec;186(23):8156-8. doi: 10.1128/JB.186.23.8156-8158.2004.

Abstract

Open reading frame sll1556 in the cyanobacterium Synechocystis sp. strain 6803 encodes a putative type II isopentenyl diphosphate (IPP) isomerase. The His(6)-tagged protein was produced in Escherichia coli and purified by Ni(2+) chromatography. The homotetrameric enzyme required NADPH, flavin mononucleotide, and Mg(2+) for activity; K(m)(IPP) was 52 microM, and k(cat)(IPP) was 0.23 s(-1).

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Carbon-Carbon Double Bond Isomerases / isolation & purification*
  • Carbon-Carbon Double Bond Isomerases / metabolism
  • Hemiterpenes
  • Kinetics
  • Synechocystis / enzymology*

Substances

  • Hemiterpenes
  • Carbon-Carbon Double Bond Isomerases
  • isopentenyldiphosphate delta-isomerase