No Transcription-Translation Feedback in Circadian Rhythm of KaiC Phosphorylation

Science. 2005 Jan 14;307(5707):251-4. doi: 10.1126/science.1102540. Epub 2004 Nov 18.

Abstract

An autoregulatory transcription-translation feedback loop is thought to be essential in generating circadian rhythms in any model organism. In the cyanobacterium Synechococcus elongatus, the essential clock protein KaiC is proposed to form this type of transcriptional negative feedback. Nevertheless, we demonstrate here temperature-compensated, robust circadian cycling of KaiC phosphorylation even without kaiBC messenger RNA accumulation under continuous dark conditions. This rhythm persisted in the presence of a transcription or translation inhibitor. Moreover, kinetic profiles in the ratio of KaiC autophosphorylation-dephosphorylation were also temperature compensated in vitro. Thus, the cyanobacterial clock can keep time independent of de novo transcription and translation processes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / metabolism*
  • Circadian Rhythm Signaling Peptides and Proteins
  • Circadian Rhythm*
  • Darkness
  • Feedback, Physiological
  • Light
  • Mutation
  • Operon
  • Phosphorylation
  • Protein Biosynthesis
  • RNA, Bacterial / metabolism
  • RNA, Messenger / metabolism
  • Recombinant Proteins / metabolism
  • Synechococcus / genetics*
  • Synechococcus / metabolism*
  • Temperature
  • Transcription, Genetic

Substances

  • Bacterial Proteins
  • Circadian Rhythm Signaling Peptides and Proteins
  • KaiA protein, cyanobacteria
  • KaiB protein, cyanobacteria
  • KaiC protein, cyanobacteria
  • RNA, Bacterial
  • RNA, Messenger
  • Recombinant Proteins