Macrophage inflammatory protein-2, neutrophil recruitment and bacterial persistence in an experimental mouse model of urinary tract infection

Microbes Infect. 2004 Nov;6(14):1326-32. doi: 10.1016/j.micinf.2004.08.008.


This study analyzed macrophage inflammatory protein-2 (MIP-2) production and neutrophil recruitment in urinary tract in response to Pseudomonas aeruginosa in an ascending model of urinary tract infection (UTI) in mice. Both planktonic and biofilm cells of P. aeruginosa were used for inducing UTI in mice. MIP-2 levels determined in urine, bladder and kidney showed maximum MIP-2 production 6 h postinfection, which correlated with neutrophil recruitment. Biofilm cells showed significantly more MIP-2 production and neutrophil recruitment. However, no correlation between bacterial numbers and neutrophil recruitment was observed in urine and kidney tissue. The role of MIP-2 and neutrophils in relation to the persistence of P. aeruginosa in the urinary tract of mice is discussed.

MeSH terms

  • Animals
  • Chemokine CXCL2
  • Chemokines / analysis
  • Chemokines / biosynthesis*
  • Chemokines / urine
  • Colony Count, Microbial
  • Female
  • Kidney / immunology
  • Kidney / microbiology
  • Mice
  • Neutrophil Infiltration*
  • Peroxidase / metabolism
  • Pseudomonas Infections / immunology
  • Pseudomonas Infections / microbiology*
  • Pseudomonas aeruginosa / growth & development*
  • Urinary Bladder / chemistry
  • Urinary Bladder / immunology
  • Urinary Bladder / microbiology
  • Urinary Tract Infections / immunology
  • Urinary Tract Infections / microbiology*
  • Urine / microbiology


  • Chemokine CXCL2
  • Chemokines
  • Cxcl2 protein, mouse
  • Peroxidase