Effects of G-CSF on cardiac remodeling after acute myocardial infarction in swine

Biochem Biophys Res Commun. 2004 Dec 24;325(4):1353-9. doi: 10.1016/j.bbrc.2004.10.149.

Abstract

We examined whether granulocyte colony-stimulating factor (G-CSF) prevents cardiac dysfunction and remodeling after myocardial infarction (MI) in large animals. MI was produced by ligation of left anterior descending coronary artery in swine. G-CSF (10 microg/kg/day, once a day) was injected subcutaneously from 24h after ligation for 7 days. Echocardiographic examination revealed that the G-CSF treatment induced improvement of cardiac function and attenuation of cardiac remodeling at 4 weeks after MI. In the ischemic region, the number of apoptotic endothelial cells was smaller and the number of vessels was larger in the G-CSF treatment group than in control group. Moreover, vascular endothelial growth factor was more abundantly expressed and Akt was more strongly activated in the ischemic region of the G-CSF treatment group than of control group. These findings suggest that G-CSF prevents cardiac dysfunction and remodeling after MI in large animals.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Granulocyte Colony-Stimulating Factor / administration & dosage*
  • Injections, Subcutaneous
  • Myocardial Infarction / complications
  • Myocardial Infarction / diagnosis
  • Myocardial Infarction / drug therapy*
  • Myocardial Infarction / physiopathology*
  • Protein-Serine-Threonine Kinases / metabolism
  • Proto-Oncogene Proteins / metabolism
  • Proto-Oncogene Proteins c-akt
  • Swine
  • Treatment Outcome
  • Vascular Endothelial Growth Factor A / metabolism
  • Ventricular Dysfunction, Left / diagnosis
  • Ventricular Dysfunction, Left / drug therapy*
  • Ventricular Dysfunction, Left / etiology
  • Ventricular Dysfunction, Left / physiopathology*
  • Ventricular Remodeling / drug effects*
  • Ventricular Remodeling / physiology*

Substances

  • Proto-Oncogene Proteins
  • Vascular Endothelial Growth Factor A
  • Granulocyte Colony-Stimulating Factor
  • Protein-Serine-Threonine Kinases
  • Proto-Oncogene Proteins c-akt