Optimization of 13C direct detection NMR methods

J Biomol NMR. 2004 Oct;30(2):175-9. doi: 10.1023/B:JNMR.0000048855.35771.11.


(13)C-detected experiments are still limited by their inherently lower sensitivity, as compared to the equivalent (1)H-detected experiments. Improving the sensitivity of (13)C detection methods remains a significant area of NMR research that may provide better means for studying large macromolecular systems by NMR. In this communication, we show that (13)C-detected experiments are less sensitive to the salt concentration of the sample solution than (1)H-detected experiments. In addition, acquisition can be started with anti-phase coherence, resulting in higher sensitivity due to the elimination of the final INEPT transfer step.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Carbon Isotopes
  • Leucine / chemistry
  • Magnetic Resonance Spectroscopy / methods*
  • Sensitivity and Specificity
  • Sodium Chloride / pharmacology
  • Solutions / chemistry


  • Carbon Isotopes
  • Solutions
  • Sodium Chloride
  • Leucine