Molecular Cloning and Characterization of a Novel Human Protein Phosphatase 2C cDNA (PP2C epsilon*)

Mol Biol Rep. 2004 Sep;31(3):197-202. doi: 10.1023/b:mole.0000043624.96006.eb.

Abstract

We have isolated a novel cDNA from the human fetal brain cDNA library with homology to the Mg2+ -dependent serine/threonine protein phosphatase 2C (PP2C) family. The cDNA is 3055 bp in length, and the predicted coding region encodes a 360-amino-acid protein, which shows 99% identity to the PP2C epsilon from rat and mouse. Then we term it human PP2C epsilon gene. The gene is mapped to chromosome 3q26.1 and contains 4 exons. RT-PCR analysis shows that the PP2C epsilon is widely expressed in human tissues and the expression levels in heart, placenta, lung, liver, kidney, and pancreas are relatively high.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / embryology
  • Chromosomes, Human, Pair 3
  • Cloning, Molecular
  • Computational Biology
  • DNA, Complementary / metabolism
  • Exons
  • Gene Library
  • Humans
  • Introns
  • MAP Kinase Signaling System
  • Magnesium / chemistry
  • Mice
  • Models, Genetic
  • Molecular Sequence Data
  • Phosphoprotein Phosphatases / biosynthesis*
  • Phosphoprotein Phosphatases / chemistry
  • Phosphoprotein Phosphatases / genetics*
  • Protein Phosphatase 2C
  • Protein Structure, Tertiary
  • RNA, Messenger / metabolism
  • Rats
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tissue Distribution

Substances

  • DNA, Complementary
  • RNA, Messenger
  • PPM1A protein, human
  • PPM1B protein, human
  • PPM1G protein, human
  • Phosphoprotein Phosphatases
  • Protein Phosphatase 2C
  • Magnesium

Associated data

  • GENBANK/AY337264