Histidine supplementation suppresses food intake and fat accumulation in rats

Nutrition. Nov-Dec 2004;20(11-12):991-6. doi: 10.1016/j.nut.2004.08.006.

Abstract

Objective: Histamine, a derivative of histidine, decreases food intake and body fat by activation of histamine neurons. Our objective was to clarify the effect of dietary histidine, in particular, on food intake and/or body fat accumulation in rats.

Methods: Male Wistar rats were assigned to one of four groups after acclimation and allowed free access to diets containing 20% casein (0% histidine), 20% casein plus 1.0% histidine, 20% casein plus 2.5% histidine, or 20% casein plus 5% histidine for 8 d.

Results: Food intake and body weight were recorded daily and compared between groups. During the experimental period, food intake decreased according to the increases in dietary histidine. There was a negative and significant (P < 0.01) correlation between dietary histidine (grams per 8 d) and retroperitoneal fat pad (grams per 100 g of body weight). Uncoupling protein-1 mRNA in brown adipose tissue increased with increases in dietary histidine.

Conclusion: Our results indicate that dietary histidine suppresses food intake and fat accumulation in rats.

MeSH terms

  • Adipose Tissue / drug effects*
  • Adipose Tissue / growth & development
  • Animals
  • Body Weight / drug effects
  • Carrier Proteins / drug effects
  • Carrier Proteins / metabolism*
  • Dietary Proteins / administration & dosage*
  • Dietary Supplements
  • Dose-Response Relationship, Drug
  • Eating / drug effects*
  • Histidine / administration & dosage*
  • Ion Channels
  • Male
  • Membrane Proteins / drug effects
  • Membrane Proteins / metabolism*
  • Mitochondrial Proteins
  • Obesity / prevention & control
  • RNA, Messenger / metabolism
  • RNA, Mitochondrial
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Uncoupling Protein 1

Substances

  • Carrier Proteins
  • Dietary Proteins
  • Ion Channels
  • Membrane Proteins
  • Mitochondrial Proteins
  • RNA, Messenger
  • RNA, Mitochondrial
  • Ucp1 protein, rat
  • Uncoupling Protein 1
  • mitochondrial messenger RNA
  • Histidine