VanE-type vancomycin-resistant Enterococcus faecalis clinical isolates from Australia

Antimicrob Agents Chemother. 2004 Dec;48(12):4882-5. doi: 10.1128/AAC.48.12.4882-4885.2004.

Abstract

Three distinct Enterococcus faecalis VanE-type isolates-BM4574, BM4575, and BM4576-obtained in Australia were studied. Expression of the resistance genes was constitutive in BM4575, probably due to a 2-bp deletion into the vanSE gene, and inducible in BM4574 and BM4576. Transcription analysis of the vanE operons suggested that the five genes were cotranscribed from an initiation site located 25 bp upstream from the ATG start codon of vanE.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Australia
  • Bacterial Proteins / genetics*
  • Chromosome Mapping
  • Codon / genetics
  • DNA Primers
  • Electrophoresis, Gel, Pulsed-Field
  • Enterococcus faecalis / drug effects*
  • Enterococcus faecalis / enzymology
  • Gram-Positive Bacterial Infections / microbiology*
  • Ligases / genetics*
  • Operon / genetics
  • Peptidoglycan / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription, Genetic
  • Vancomycin Resistance / genetics*

Substances

  • Bacterial Proteins
  • Codon
  • DNA Primers
  • Peptidoglycan
  • Ligases
  • VanE protein, Enterococcus faecalis