New modules for the repeated internal and N-terminal epitope tagging of genes in Saccharomyces cerevisiae

Yeast. 2005 Jan 15;22(1):1-12. doi: 10.1002/yea.1187.


Epitope tagging is a powerful method for the rapid analysis of protein function. In Saccharomyces cerevisiae epitope tags are introduced easily into chromosomal loci by homologous recombination using a simple PCR-based strategy. Although quite a number of tools exist for C-terminal tagging as well as N-terminal tagging of proteins expressed by heterologous promoters, there are only very limited possibilities to tag proteins at the N-terminus and retain the endogenous expression level. Furthermore, no PCR-templates for internal tagging have been reported. Here we describe new modules that are suitable for both the repeated N-terminal and internal tagging of proteins, leaving their endogenous promoters intact. The tags include 6xHA, 9xMyc, yEGFP, TEV-GST-6xHIS, ProtA, TEV-ProtA and TEV-ProtA-7xHIS in conjunction with different heterologous selection markers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromosomes, Fungal
  • Epitope Mapping
  • Genes, Fungal*
  • Molecular Sequence Data
  • Plasmids
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins / immunology
  • Saccharomyces cerevisiae Proteins / physiology*


  • Saccharomyces cerevisiae Proteins