An Unusually High Level of Quinolone Resistance Associated With Type II Topoisomerase Mutations in Quinolone Resistance-Determining Regions of Aeromonas Caviae Isolated From Diarrhoeal Patients

Res Microbiol. 2004 Dec;155(10):827-9. doi: 10.1016/j.resmic.2004.06.008.

Abstract

We examined 158 strains belonging to different Aeromonas species isolated from hospitalized acute diarrhoea cases for susceptibility to quinolones. Compared to other species, a high percentage of the A. caviae strains were resistant to ciprofloxacin and norfloxacin. Based on MIC values, 6 A. caviae strains were selected and the nucleotide sequences for the quinolone-resistant-determining regions (QRDRs) of gyrA, gyrB and parC genes were analysed. In resistant strains, double mutations (Ser(83)-->Ile and Asp(87)-->Asn) and a single mutation (Ser(80)-->Ile) were detected in the QRDR of gyrA and parC, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aeromonas / drug effects*
  • Aeromonas / enzymology
  • Aeromonas / genetics
  • Anti-Infective Agents / pharmacology*
  • Base Sequence
  • Ciprofloxacin / pharmacology*
  • DNA Gyrase / genetics*
  • DNA Topoisomerase IV / genetics*
  • DNA, Bacterial / genetics
  • Diarrhea / microbiology
  • Drug Resistance, Bacterial / genetics
  • Gram-Negative Bacterial Infections / microbiology*
  • Humans
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Norfloxacin / pharmacology*

Substances

  • Anti-Infective Agents
  • DNA, Bacterial
  • Ciprofloxacin
  • DNA Topoisomerase IV
  • DNA Gyrase
  • Norfloxacin

Associated data

  • GENBANK/AY377878
  • GENBANK/AY377879
  • GENBANK/AY377880
  • GENBANK/AY377881
  • GENBANK/AY377882
  • GENBANK/AY377883
  • GENBANK/AY377884
  • GENBANK/AY377885
  • GENBANK/AY377886
  • GENBANK/AY377887
  • GENBANK/AY377888
  • GENBANK/AY377889