The symbiotic defect in a Sinorhizobium meliloti lipopolysaccharide mutant can be overcome by expression of other surface polysaccharides

Res Microbiol. 2004 Dec;155(10):855-60. doi: 10.1016/j.resmic.2004.06.012.

Abstract

In this work we have examined the extent of functional complementation in symbiosis among different Sinorhizobium meliloti surface polysaccharides including lipopolysaccharide (LPS). We show that a symbiotic deficiency associated with an LPS defect can be reversed by appropriate expression of other surface polysaccharides such as galactoglucan (EPSII) and a particular form of capsular polysaccharide (KdoPS). It is noteworthy that, while succinoglycan EPSI and LPS cannot functionally substitute for each other, they can both be replaced by the same common set of polysaccharides (i.e., EPSII/KdoPS). The complex pattern of functional complementation in symbiosis among S. meliloti surface polysaccharides was shown to be different in Medicago truncatula compared to that previously reported for M. sativa.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Southern
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • Lipopolysaccharides / metabolism*
  • Medicago sativa / physiology
  • Mutagenesis, Site-Directed
  • Nitrogen Fixation / physiology
  • Polysaccharides, Bacterial / biosynthesis*
  • Polysaccharides, Bacterial / physiology
  • Recombinant Proteins / metabolism
  • Sinorhizobium meliloti / genetics
  • Sinorhizobium meliloti / metabolism
  • Sinorhizobium meliloti / physiology*
  • Symbiosis / physiology

Substances

  • DNA, Fungal
  • Lipopolysaccharides
  • Polysaccharides, Bacterial
  • Recombinant Proteins