Therapeutic dendritic-cell vaccine for chronic HIV-1 infection

Nat Med. 2004 Dec;10(12):1359-65. doi: 10.1038/nm1147. Epub 2004 Nov 28.


We present the results of a preliminary investigation of the efficacy of a therapeutic dendritic cell (DC)-based vaccine for HIV-1. We immunized 18 chronically HIV-1-infected and currently untreated individuals showing stable viral loads for at least 6 months with autologous monocyte-derived DCs loaded with autologous aldrithiol-2-inactivated HIV-1. Plasma viral load levels were decreased by 80% (median) over the first 112 d following immunization. Prolonged suppression of viral load of more than 90% was seen in 8 individuals for at least 1 year. The suppression of viral load was positively correlated with HIV-1-specific interleukin-2 or interferon-gamma-expressing CD4(+) T cells and with HIV-1 gag-specific perforin-expressing CD8(+) effector cells, suggesting that a robust virus-specific CD4(+) T-helper type 1 (T(H)1) response is required for inducing and maintaining virus-specific CD8(+) effectors to contain HIV-1 in vivo. The results suggest that inactivated whole virus-pulsed DC vaccines could be a promising strategy for treating people with chronic HIV-1 infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2,2'-Dipyridyl / analogs & derivatives*
  • AIDS Vaccines / immunology
  • AIDS Vaccines / therapeutic use*
  • Adult
  • Brazil
  • CD4-Positive T-Lymphocytes / metabolism
  • CD8-Positive T-Lymphocytes / metabolism
  • Dendritic Cells / immunology*
  • Disulfides
  • Enzyme-Linked Immunosorbent Assay
  • Flow Cytometry
  • HIV Infections / drug therapy*
  • HIV Infections / prevention & control*
  • HIV-1 / immunology*
  • Humans
  • Interferon-gamma / metabolism
  • Interleukin-2 / blood
  • Membrane Glycoproteins / metabolism
  • Perforin
  • Pore Forming Cytotoxic Proteins
  • T-Lymphocytes, Helper-Inducer / immunology
  • Time Factors
  • Viral Load


  • AIDS Vaccines
  • Disulfides
  • Interleukin-2
  • Membrane Glycoproteins
  • Pore Forming Cytotoxic Proteins
  • Perforin
  • 2,2'-dipyridyl disulfide
  • 2,2'-Dipyridyl
  • Interferon-gamma