Polymorphism of the thymidylate synthase gene and thymidylate synthase levels in colon cancer cell lines and different tissues of colorectal cancer patients

R Mauritz; I J Beumer; S Marsh; H L McLeod; K Smid; C J van Groeningen; H M Pinedo; G J Peters

doi:10.1081/NCN-200027634

Polymorphism of the thymidylate synthase gene and thymidylate synthase levels in colon cancer cell lines and different tissues of colorectal cancer patients

Nucleosides Nucleotides Nucleic Acids. 2004 Oct;23(8-9):1381-4. doi: 10.1081/NCN-200027634.

Authors

R Mauritz¹, I J Beumer, S Marsh, H L McLeod, K Smid, C J van Groeningen, H M Pinedo, G J Peters

Affiliation

¹ Department of Medical Oncology, VU University Medical Center, Amsterdam, The Netherlands.

PMID: 15571263
DOI: 10.1081/NCN-200027634

Abstract

In a panel of 18 colon cancer cell lines we found that the thymidylate synthase (TS) genotype was related to TS enzyme activity, but not to TS protein and mRNA levels. In addition, no relation with drug sensitivity was observed. TS genotyping of different tissues from 78 colorectal cancer patients revealed a high level of homology in polymorphic status between normal and malignant tissues and the heterozygous genotype to be the most frequent.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antimetabolites, Antineoplastic / pharmacology
Cell Line, Tumor
Colonic Neoplasms / enzymology*
Colonic Neoplasms / genetics*
Drug Resistance, Neoplasm
Fluorouracil / pharmacology
Genotype
Heterozygote
Humans
Polymorphism, Genetic*
RNA, Messenger / metabolism
Thymidylate Synthase / biosynthesis*
Thymidylate Synthase / genetics*
Tissue Distribution

Substances

Antimetabolites, Antineoplastic
RNA, Messenger
Thymidylate Synthase
Fluorouracil