Comparison of mRNA expression levels determined with TaqMan and competitive template RT-PCR

Nucleosides Nucleotides Nucleic Acids. 2004 Oct;23(8-9):1471-4. doi: 10.1081/NCN-200027689.

Abstract

Two methods for measurement of thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPD) mRNA expression were compared. Although the relative mRNA levels compared to beta-actin measured with competitive template RT-PCR were different from the data obtained with a TaqMan based PCR, a significant correlation between the two assays was found.

Publication types

  • Comparative Study

MeSH terms

  • Actins / metabolism
  • Antimetabolites, Antineoplastic / pharmacology
  • Colorectal Neoplasms / metabolism
  • Dihydrouracil Dehydrogenase (NADP) / metabolism
  • Fluorouracil / pharmacology*
  • Gene Expression Regulation
  • Humans
  • Polymerase Chain Reaction / methods*
  • RNA, Messenger / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Thymidylate Synthase / metabolism

Substances

  • Actins
  • Antimetabolites, Antineoplastic
  • RNA, Messenger
  • Dihydrouracil Dehydrogenase (NADP)
  • Thymidylate Synthase
  • Fluorouracil