Abstract
Two methods for measurement of thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPD) mRNA expression were compared. Although the relative mRNA levels compared to beta-actin measured with competitive template RT-PCR were different from the data obtained with a TaqMan based PCR, a significant correlation between the two assays was found.
MeSH terms
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Actins / metabolism
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Antimetabolites, Antineoplastic / pharmacology
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Colorectal Neoplasms / metabolism
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Dihydrouracil Dehydrogenase (NADP) / metabolism
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Fluorouracil / pharmacology*
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Gene Expression Regulation
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Humans
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Polymerase Chain Reaction / methods*
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RNA, Messenger / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Thymidylate Synthase / metabolism
Substances
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Actins
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Antimetabolites, Antineoplastic
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RNA, Messenger
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Dihydrouracil Dehydrogenase (NADP)
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Thymidylate Synthase
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Fluorouracil