Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene

Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2624-8. doi: 10.1073/pnas.89.7.2624.


Molecular gene transfer techniques have been used to engineer the fatty acid composition of Brassica rapa and Brassica napus (canola) oil. Stearoyl-acyl carrier protein (stearoyl-ACP) desaturase (EC catalyzes the first desaturation step in seed oil biosynthesis, converting stearoyl-ACP to oleoyl-ACP. Seed-specific antisense gene constructs of B. rapa stearoyl-ACP desaturase were used to reduce the protein concentration and enzyme activity of stearoyl-ACP desaturase in developing rapeseed embryos during storage lipid biosynthesis. The resulting transgenic plants showed dramatically increased stearate levels in the seeds. A continuous distribution of stearate levels from 2% to 40% was observed in seeds of a transgenic B. napus plant, illustrating the potential to engineer specialized seed oil compositions.

MeSH terms

  • Base Sequence
  • Brassica / genetics*
  • Brassica / growth & development
  • Cloning, Molecular
  • DNA / genetics
  • Gene Expression
  • Genetic Engineering
  • Kanamycin / pharmacology
  • Mixed Function Oxygenases / genetics*
  • Mixed Function Oxygenases / metabolism
  • Molecular Sequence Data
  • Plant Oils / metabolism*
  • RNA, Antisense
  • RNA, Messenger / genetics
  • Stearates / metabolism


  • Plant Oils
  • RNA, Antisense
  • RNA, Messenger
  • Stearates
  • Kanamycin
  • DNA
  • Mixed Function Oxygenases
  • acyl-(acyl-carrier-protein)desaturase

Associated data

  • GENBANK/X60978