Analyses of peripheral blood mononuclear cells in operational tolerance after pediatric living donor liver transplantation

Am J Transplant. 2004 Dec;4(12):2118-25. doi: 10.1111/j.1600-6143.2004.00611.x.


Operational tolerance (graft acceptance in an immunosuppression (IS)-free environment) after living-donor liver transplantation (LDLT) could occur by our elective protocol in some patients. There is, nevertheless, no reliable parameter to monitor patients who may discontinue IS without a risk of rejection. To identify such parameters, we systemically phenotyped peripheral blood mononuclear cells from operationally tolerant patients. An increase was observed in the frequency of CD4+CD25high+ cells, B cells and Vdelta1/Vdelta2 gammadeltaT-cells ratio in operationally tolerant patients (Gr-tol; n = 12), compared with those from age-matched volunteers (Gr-vol; n = 24) or patients on IS (Gr-IS; n = 19). The frequency of NK cells was decreased in Gr-tol, compared with those in Gr-IS or Gr-vol. The frequency of NKT cells was decreased after LDLT, compared with that in Gr-vol. Although the contribution of those subsets to the tolerant state remains elusive, the results may provide important clues for reliable indicators of tolerance after LDLT.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Antigens, CD / blood
  • Child
  • Child, Preschool
  • Female
  • Flow Cytometry
  • Graft Survival / immunology
  • Graft Survival / physiology*
  • Humans
  • Infant
  • Killer Cells, Natural / immunology
  • Leukocytes, Mononuclear / physiology*
  • Liver Transplantation / immunology*
  • Living Donors*
  • Male
  • Retrospective Studies
  • T-Lymphocytes / immunology*


  • Antigens, CD