Effects of immunomodulators on ecto-5'-nucleotidase activity on blood mononuclear cells in vitro

Scand J Immunol. 1992 Apr;35(4):407-13. doi: 10.1111/j.1365-3083.1992.tb02875.x.


In this study the effects of immunomodulators on the ecto-5'-nucleotidase (ecto-5'-NT) activity on blood mononuclear cells (BMC) were examined in vitro. Interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) decreased the level of ecto-5'-NT activity on BMC whereas prostaglandin E2 (PGE2) increased the ecto-5'-NT level. All three immunomodulators influenced the ecto-5'-NT activity of isolated monocytes whereas only IL-4 and PGE2 had an effect on the enzyme level on isolated lymphocytes. The effect was dependent upon protein synthesis. The effect was dose dependent: IL-4 was effective at concentrations down to 0.5 U/ml, IFN-gamma down to 40 U/ml and PGE2 at nanomolar concentrations. These data indicate that immunomodulators may also take part in the regulation of ecto-5'-NT activity on BMC in vivo. BMC from 7 patients with different immunodeficiency syndromes showed decreased ecto-5'-NT activity on freshly isolated cells. However, following culture ecto-5'-NT activity was increased above the level found on freshly isolated BMC from healthy persons. On BMC from 3 patients with hypogammaglobulinaemia, the effect of IL-4 on the level of ecto-5'-NT activity was identical to that found on BMC from healthy donors, whereas PGE2 increased ecto-5'-NT activity on BMC from only 1 of the 3 patients investigated. The decreased ecto-5'-NT activity of BMC from patients with immunodeficiency may thus be due to a defective regulation of ecto-5'-NT activity in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5'-Nucleotidase / metabolism*
  • Adjuvants, Immunologic / pharmacology*
  • Agammaglobulinemia / enzymology
  • Dinoprostone / pharmacology
  • Humans
  • In Vitro Techniques
  • Interferon-gamma / pharmacology
  • Interleukin-4 / pharmacology
  • Leukocytes, Mononuclear / enzymology*


  • Adjuvants, Immunologic
  • Interleukin-4
  • Interferon-gamma
  • 5'-Nucleotidase
  • Dinoprostone